The purpose of this study is to evaluate histologically the resorption and tissue response of various resorbable membranes used for guided tissue regeneration procedures, using a subcutaneous model on the dorsal surface of the rat. In this study, 12 Sprague-Dawley male rats(mean BW 150gm) were used and the commercially available materials included dense collagen membrane, freeze-dried bovine dura mater, loose collagen membrane, PLA/PLGA membrane. Animals were sacrificed at 3, 6 and 8 weeks after implantation of various resorbable membranes. Specimens were prepared with Hematoxylin-Eosin stain for light microscopic evaluation.
The results of this study were as follows:
1. Resorption : Loose collagen membrane group was resorted most rapidly. Dense collagen membrane group and freeze-dried bovine dura mater group were rarely resorted.
2. Inflammatory reactions : PLA/PLGA membrane group showed persistent and severe inflammatory reactions for 3 to 8 weeks. Moderate inflammatory reactions and the ectopic formation of calcified material were observed in dense collagen membrane group. Freeze-dried bovine dura mater group and loose collagen membrane group showed mild inflammatory reactions
3. In PLA/PLGA membrane group, multinucleated giant cells by foreign body reactions were observed.
In conclusion, the resorption of freeze-dried bovine dura mater didn´t happen for 3-6weeks, which showed the best biocompatibility. Therefore, freeze-dried bovine dura mater was considered proper resorbable membrane for guided tissue regeneration.
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